| Title | A New Specialization in Astrocytes: Glutamate- and Ammonia-Induced Nuclear Size Changes |
| Authors | Yang, Chun Zhang Li, Hui Li Zhou, Ye Chai, Rui Chao Zhao, Rui Dong, Yan Xu, Zhen Yu Lau, Lok Ting Zhang Yingge Teng, Junlin Chen, Jianguo Yu, Albert Cheung Hoi |
| Affiliation | Peking Univ, Neurosci Res Inst, Sch Basic Med Sci,Hlth Sci Ctr, Key Lab Neurosci,Minist Educ,Minist Publ Hlth,Dep, Beijing 100191, Peoples R China. Beijing Inst Pharmacol & Toxicol, Beijing, Peoples R China. Peking Univ, Key Lab Cell Proliferat & Differentiat, State Key Lab Biomembrane & Membrane Bioengn, Coll Life Sci,Minist Educ, Beijing 100191, Peoples R China. Peking Univ, Ctr Theoret Biol, Beijing 100191, Peoples R China. Peking Univ, Ctr Infect Dis, Beijing 100191, Peoples R China. Peking Univ, Lab Translat Med, Inst Syst Biomed, Beijing 100191, Peoples R China. Peking Univ, Neurosci Res Inst, Sch Basic Med Sci,Hlth Sci Ctr, Key Lab Neurosci,Minist Educ,Minist Publ Hlth,Dep, 38 Xue Yuan Rd, Beijing 100191, Peoples R China. |
| Keywords | astrocyte nuclear swelling cytoplasmic swelling glutamate ammonia atomic force microscopy MESSENGER-RNA EXPRESSION NECROSIS-FACTOR-ALPHA GLIAL-CELLS HEPATIC-ENCEPHALOPATHY SYNAPTIC-TRANSMISSION WATER PERMEABILITY XENOPUS OOCYTES MECHANISMS TRANSPORT CYTOSKELETON |
| Issue Date | 2011 |
| Publisher | journal of neuroscience research |
| Citation | JOURNAL OF NEUROSCIENCE RESEARCH.2011,89,(12,SI),2041-2051. |
| Abstract | We observed nuclear swelling in glutamate (Glu)-treated astrocytes that was concomitant with but independent of astrocytic cell swelling. We confirmed Glu-induced nuclear swelling with nuclei isolated from astrocytes. Ammonia is metabolically related to Glu and could induce a nuclear swelling in intact astrocytes but shrinkage in isolated nuclei. Other compounds such as glutamine, aspartate, taurine, glycine, and ATP did not cause any nuclear swelling in isolated nuclei of astrocytes. Surprisingly, Glu and ammonia did not induce nuclear swelling in microglia, C6, HEK 293, or Hep G2 cell lines in cultures and their isolated nuclei. The Glu- and ammonia-induced nuclear size changes appear to be a specific response of astrocytes to these two closely related metabolic compounds. (C) 2011 Wiley-Liss, Inc. |
| URI | http://hdl.handle.net/20.500.11897/237498 |
| ISSN | 0360-4012 |
| DOI | 10.1002/jnr.22657 |
| Indexed | SCI(E) |
| Appears in Collections: | 医学部待认领 生命科学学院 细胞增殖分化调控机理研究教育部重点实验室 |
