| Title | Lanthanum chloride bidirectionally influences calcification in bovine vascular smooth muscle cells |
| Authors | Zhao, Wen-Hua Gou, Bao-Di Zhang, Tian-Lan Wang, Kui |
| Affiliation | Peking Univ, Dept Biol Chem, Sch Pharmaceut Sci, Beijing 100191, Peoples R China. Capital Med Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100069, Peoples R China. Peking Univ, Dept Biol Chem, Sch Pharmaceut Sci, 38 Xueyuan Rd, Beijing 100191, Peoples R China. |
| Keywords | VASCULAR SMOOTH MUSCLE CELLS LANTHANUM CHLORIDE CALCIUM PHOSPHATE DEPOSITION CALCIUM-SENSING RECEPTOR APOPTOSIS CALCIUM-SENSING RECEPTOR NEPHROGENIC SYSTEMIC FIBROSIS PHOSPHATE CRYSTALS ATHEROSCLEROSIS GADOLINIUM CARBONATE DEPOSITION APOPTOSIS CYTOTOXICITY MECHANISMS |
| Issue Date | 2012 |
| Publisher | 细胞生物化学杂志 |
| Citation | JOURNAL OF CELLULAR BIOCHEMISTRY.2012,113,(5),1776-1786. |
| Abstract | Vascular calcification (VC) is frequent prevalence in patients with chronic kidney disease (CKD) and atherosclerosis. Lanthanum carbonate is used as an orally administered phosphate-binding agent to reduce the gastrointestinal absorption of phosphate and ameliorate VC in advanced CKD. In this study, we used bovine vascular smooth muscle cells as a model VC in vitro and studied the effects of lanthanum chloride on calcium deposition. Exposure of cells to LaCl3 at the concentration of 0.1 mu M suppressed the beta-glycerophosphate-induced alkaline phosphatase activity and calcium deposition. Furthermore, LaCl3 upregulated the beta-glycerophosphate-suppressed expression of calcium-sensing receptor. In contrast to the inhibitory effect of LaCl3 on calcium deposition, higher level lanthanum (50 mu M) was found to promote immediately precipitation of calcium phosphate in cell culture medium. At this concentration, LaCl3 was found to induce cell apoptosis which involves caspases-9 and -3. These data indicate that the promotory effect of LaCl3 on calcium deposition is likely mediated by induction of apoptosis. Our in vitro findings do suggest that, in the context of raised lanthanum, greater attention should be paid to potential toxic effects associated to the use of lanthanide-based drugs. J. Cell. Biochem. 113: 17761786, 2012. (C) 2011 Wiley Periodicals, Inc. |
| URI | http://hdl.handle.net/20.500.11897/235483 |
| ISSN | 0730-2312 |
| DOI | 10.1002/jcb.24049 |
| Indexed | SCI(E) |
| Appears in Collections: | 药学院 |
