| Title | In vitro and in vivo evaluation of oridonin-loaded long circulating nanostructured lipid carriers |
| Authors | Jia, Lejiao Shen, Jingyi Zhang, Dianrui Duan, Cunxian Liu, Guangpu Zheng, Dandan Tian, Xiaona Liu, Yue Zhang, Qiang |
| Affiliation | Shandong Univ, Dept Pharmaceut, Coll Pharm, Jinan 250012, Peoples R China. Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100083, Peoples R China. Shandong Univ, Dept Pharmaceut, Coll Pharm, 44 Wenhua Xilu, Jinan 250012, Peoples R China. |
| Keywords | Oridonin Nanostructured lipid carriers Long circulating In vitro release properties Pharmacokinetics NANOPARTICLES NLC DELIVERY CELLS BIODISTRIBUTION STEARATE BRAIN SLN |
| Issue Date | 2012 |
| Publisher | 国际生物大分子杂志 |
| Citation | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES.2012,50,(3),523-529. |
| Abstract | The purpose of this study was to develop poly(ethylene glycol)-coated nanostructured lipid carriers (PEG-NLC) for parenteral delivery of oridonin (ORI) to prolong drug circulation time in blood. Oridonin-loaded PEG-NLC (ORI-PEG-NLC) consisting of PEG(2000)-stearate, glycerol monostearate and medium chain triglycerides were prepared by emulsion-evaporation and low temperature-solidification technique. Oridonin-loaded NLC (ORI-NLC) were also prepared as control. ORI-PEG-NLC were observed by transmission election microscope and the morphology was in rotiform shape. The mean particle size of ORI-PEG-NLC was 329.2 nm and entrapment efficacy was 71.18%. The results of differential scanning calorimetry and X-ray diffraction revealed a low-crystalline structure of ORI and verified the incorporation of ORI into the nanoparticles. In vitro drug release of ORI-PEG-NLC exhibited biphasic drug release patterns with burst release initially and prolonged release afterwards. Pharmacokinetic analysis showed that the mean residence time of ORI-PEG-NLC was prolonged and AUC (area under tissue concentration-time curve) value was also improved compared with ORI-NLC and ORI solution. In conclusion, ORI-PEG-NLC could be a potential carrier to get prolonged retention time of oridonin in blood. (C) 2012 Elsevier B.V. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/233870 |
| ISSN | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2012.01.024 |
| Indexed | SCI(E) |
| Appears in Collections: | 药学院 天然药物与仿生药物国家重点实验室 |
