| Title | Possible novel roles of poly(rC)-binding protein 1 in SH-SY5Y neurocytes: an analysis using a dynamic Bayesian network |
| Authors | Huo, Li-Rong Liang, Jian-Tao Zou, Jun-Hua Wang, Lan-Ying Li, Qi Wang, Xiao-Min |
| Affiliation | Capital Med Univ, Dept Physiol, Key Lab Neurodegenerat Disorders, Minist Educ, Beijing 100069, Peoples R China. Peking Univ, Ctr Med Genet, Beijing 100083, Peoples R China. Capital Med Univ, Xuan Wu Hosp, Beijing 100053, Peoples R China. Genminix Informat Ltd Co, Shanghai 201210, Peoples R China. |
| Keywords | PCBP1 RNA interference overexpression Bayesian network SPERMIDINE/SPERMINE N-1-ACETYLTRANSFERASE 3'-UNTRANSLATED REGION SIGNAL-TRANSDUCTION MESSENGER-RNA EXPRESSION CANCER IDENTIFICATION TRANSCRIPTS LOCALIZATION MECHANISM |
| Issue Date | 2012 |
| Publisher | 神经科学通报 |
| Citation | NEUROSCIENCE BULLETIN.2012,28,(3),282-290. |
| Abstract | Poly(rC)-binding protein 1 (PCBP1) belongs to the heterogeneous nuclear ribonucleoprotein family and participates in transcriptional and translational regulation. Previous work has identified transcripts targeted by both knockdown and overexpression of PCBP1 in SH-SY5Y neuroblastoma cells using a microarray or ProteomeLab (TM) protein fractionation 2-dimensions (PF-2D) and quadrupole time-of-flight mass spectrometer. The present study aimed to further determine whether these altered transcripts from major pathways (such as Wnt signaling, TGF-beta signaling, cell cycling, and apoptosis) and two other genes, H2AFX and H2BFS (screened by PF-2D), have spatial relationships. The genes were studied by qRT-PCR, and dynamic Bayesian network analysis was used to rebuild the coordination network of these transcripts. PCBP1 controlled the expression or activity of the seven transcripts. Moreover, PCBP1 indirectly regulated MAP2K2, FOS, FST, TP53 and WNT7B through H2AFX or regulated these genes through SAT. In contrast, TP53 and WNT7B are regulated by other genes. The seven transcripts and PCBP1 are closely associated in a spatial interaction network. |
| URI | http://hdl.handle.net/20.500.11897/232999 |
| ISSN | 1673-7067 |
| DOI | 10.1007/s12264-012-1242-6 |
| Indexed | SCI(E) 中国科技核心期刊(ISTIC) 中国科学引文数据库(CSCD) |
| Appears in Collections: | 医学部待认领 |
