| Title | Application of a nanotechnology antimicrobial spray to prevent lower urinary tract infection: a multicenter urology trial |
| Authors | He, Wei Wang, Dongmin Ye, Zhangqun Qian, Weihong Tao, Yan Shi, Xiaofeng Liu, Ling Chen, Jin Qiu, Ling Wan, Peng Jia, Xiaojun Li, Xia Gao, Caixia Ma, Xuexia Wen, Biyan Chen, Nianzhen Li, Ping Ren, Zhengzheng Lan, Li Li, Siyi Zuo, Yi Zhang, Hua Ma, Liming Zhang, Yueping Li, Zhicong Su, Weiping Yang, Qing Chen, Qingli Wang, Xuejing Ye, Zhenni Chen, J. P. Loo, Wings T. Y. Chow, Louis W. C. Yip, Adrian Y. S. Ng, Elizabeth L. Y. Cheung, Mary N. B. Wang, Zhiping |
| Affiliation | Univ Hong Kong, Sch Chinese Med, Hong Kong, Hong Kong, Peoples R China. UNIMED Med Inst, Hong Kong, Hong Kong, Peoples R China. Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp Affiliated, Dept Urol, Wuhan, Hubei, Peoples R China. Lanzhou Univ, Hosp 2, Inst Urol, Lanzhou 730000, Peoples R China. Wuhan Gen Hosp Guangzhou Mil Reg, Guangzhou, Guangdong, Peoples R China. Sichuan Univ, W China Hosp, Chengdu, Sichuan, Peoples R China. Third Mil Med Univ, Daping Hosp, Chongqing, Peoples R China. Guangzhou Med Univ, Affiliated Hosp 1, Guangzhou, Guangdong, Peoples R China. Second Mil Med Univ, Changhai Hosp, Shanghai, Peoples R China. Peking Univ, Peoples Hosp, Beijing 100871, Peoples R China. Sun Yat Sen Univ, Affiliated Hosp 3, Guangzhou, Guangdong, Peoples R China. Xi An Jiao Tong Univ, Second Hosp, Xian 710049, Peoples R China. Sun Yat Sen Univ, Affiliated Hosp 2, Guangzhou 510275, Guangdong, Peoples R China. Cent S Univ, Xiangya Hosp, Changsha, Hunan, Peoples R China. Nanjing Univ, Sch Med, Affiliated Hosp, Nanjing Drum Tower Hosp, Nanjing, Jiangsu, Peoples R China. Nanjing Med Univ, Huaian Hosp Affiliated 1, Nanjing, Jiangsu, Peoples R China. Sun Yat Sen Univ, Affiliated Hosp 1, Guangzhou 510275, Guangdong, Peoples R China. Guangzhou Univ Tradit Chinese Med, Affiliated Hosp 2, Guangzhou, Guangdong, Peoples R China. So Med Univ, Affiliated Hosp 1, Nanfang Hosp, Guangzhou, Guangdong, Peoples R China. Nantong Univ, Affiliated Hosp, Nantong, Jiangsu, Peoples R China. Foshan Hosp Tradit Chinese Med, Guangzhou, Guangdong, Peoples R China. Guangzhou First Municipal Peoples Hosp, Guangzhou, Guangdong, Peoples R China. Gen Hosp Guangzhou Mil Command PLA, Guangzhou, Guangdong, Peoples R China. Nanjing Med Univ, Affiliated Hosp 1, Nanjing, Jiangsu, Peoples R China. Kunming Med Coll, Affiliated Hosp 2, Kunming, Yunnan, Peoples R China. |
| Keywords | CATHETER-ASSOCIATED UROPATHOGENS BIOFILM FORMATION INDWELLING CATHETER BACTERIAL BIOFILMS ESCHERICHIA-COLI CLINICAL-TRIAL IN-VITRO CARE PATHOGENESIS MATRIX |
| Issue Date | 2012 |
| Citation | JOURNAL OF TRANSLATIONAL MEDICINE.2012/9/19,10. |
| Abstract | Background: Catheter-associated urinary tract infection (CAUTI) is a common nosocomial device-associated infection. It is now recognized that the high infection rates were caused by the formation of biofilm on the surface of the catheters that decreases the susceptibility to antibiotics and results in anti-microbial resistance. In this study, we performed an in vitro test to explore the mechanism of biofilm formation and subsequently conducted a multi-center clinical trial to investigate the efficacy of CAUTI prevention with the application of JUC, a nanotechnology antimicrobial spray. Methods: Siliconized latex urinary catheters were cut into fragments and sterilized by autoclaving. The sterilized sample fragments were randomly divided into the therapy and control group, whereby they were sprayed with JUC and distilled water respectively and dried before use. The experimental standard strains of Escherichia coli (E. coli) were isolated from the urine samples of patients. At 16 hours and 7 days of incubation, the samples were extracted for confocal laser scanning microscopy. A total of 1,150 patients were accrued in the clinical study. Patients were randomized according to the order of surgical treatment. The odd array of patients was assigned as the therapy group (JUC), and the even array of patients was assigned as the control group (normal saline). Results: After 16 hours of culture, bacterial biofilm formed on the surface of sample fragments from the control group. In the therapy group, no bacterial biofilm formation was observed on the sample fragments. No significant increase in bacterial colony count was observed in the therapy group after 7 days of incubation. On the 7th day of catheterization, urine samples were collected for bacterial culture before extubation. Significant difference was observed in the incidence of bacteriuria between the therapy group and control group (4.52% vs. 13.04%, p < 0.001). Conclusions: In this study, the effectiveness of JUC in preventing CAUTI in a hospital setting was demonstrated in both in vitro and clinical studies. |
| URI | http://hdl.handle.net/20.500.11897/230756 |
| ISSN | 1479-5876 |
| DOI | 10.1186/1479-5876-10-S1-S14 |
| Indexed | SCI(E) CPCI-S(ISTP) |
| Appears in Collections: | 人民医院 |
