| Title | Identification of UHRF1/2 as new N-methylpurine DNA glycosylase-interacting proteins |
| Authors | Liang, Chao Zhang, Xueli Song, Shanshan Tian, Chunyan Yin, Yuxin Xing, Guichun He, Fuchu Zhang, Lingqiang |
| Affiliation | Anhui Med Univ, Dept Cell Biol, Hefei 230032, Anhui, Peoples R China. Beijing Inst Radiat Med, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 100850, Peoples R China. Univ So Calif, Dept Mol Microbiol & Immunol, Los Angeles, CA 90033 USA. Peking Univ, Dept Pathol, Sch Basic Med Sci, Beijing 100191, Peoples R China. |
| Keywords | MPG UHRF1 UHRF2 IP/MS Protein interaction BASE EXCISION-REPAIR CELL-CYCLE ARREST ALKYLATING-AGENTS CANCER METHYLATION SENSITIVITY ICBP90 TARGET DNMT1 LINKS |
| Issue Date | 2013 |
| Publisher | 生物化学与生物物理学研究通讯 |
| Citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2013,433,(4),415-419. |
| Abstract | N-methylpurine DNA glycosylase (MPG), a DNA repair enzyme, functions in the DNA base excision repair (BER) pathway. Aberrant over-expression of MPG in various cancers suggests an important role of MPG in carcinogenesis. Identification of MPG-interacting proteins will help to dissect the molecular link between MPG and cancer development. In the present study, using immunoprecipitation coupled with mass spectrometry (IP/MS), we screened ubiquitin-like, containing PHD and RING finger domains 1 (UHRF1), an essential protein required for the maintenance of DNA methylation, as a MPG-interacting protein. Endogenous co-immunoprecipitation assay in cancer cells confirmed that UHRF1 interacted with MPG in a p53 status-independent manner. Confocal microscopy showed that endogenous MPG and UHRF1 were co-localized in the nucleoplasm. Furthermore, co-immunoprecipitation assay indicated that UHRF2, the homolog of UHRF1, could also interact with MPG. These results show that MPG and the UHRF family of proteins interact, thus providing a functional linkage between MPG and UHRF1/2. (C) 2013 Elsevier Inc. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/225360 |
| ISSN | 0006-291X |
| DOI | 10.1016/j.bbrc.2013.02.126 |
| Indexed | SCI(E) |
| Appears in Collections: | 基础医学院 |
