| Title | VEGF111b, a new member of VEGFxxxb isoforms and induced by mitomycin C, inhibits angiogenesis |
| Authors | Gu, Fang Li, Xiuli Kong, Jian Pan, Bing Sun, Min Zheng, Lemin Yao, Yuanqing |
| Affiliation | Chinese Peoples Liberat Army Gen Hosp, Dept Obstet & Gynecol, Beijing, Peoples R China. Peking Univ, Inst Cardiovasc Sci,Hlth Minist, Key Lab Cardiovasc Mol Biol & Regulatory Peptides, Key Lab Mol Cardiovasc Sci,Educ Minist,Hlth Sci C, Beijing 100871, Peoples R China. Peking Univ, Key Lab Cardiovasc Mol Biol & Regulatory Peptides, Sch Basic Med Sci,Educ Minist,Inst Syst Biomed, Key Lab Mol Cardiovasc Sci,Hlth Sci Ctr,Hlth Mini, Beijing 100871, Peoples R China. Capital Med Univ, Beijing Chaoyang Hosp, Dept Hepatobiliary Surg, Beijing, Peoples R China. Fourth Mil Med Univ, Tangdu Hosp, Dept Obstet & Gynecol, Xian 710032, Peoples R China. |
| Keywords | VEGFxxxb Anti-angiogenesis Splice variant VEGFR-2 ENDOTHELIAL GROWTH-FACTOR SPLICE VARIANT IN-VIVO VEGF(165)B NEOVASCULARIZATION NEUROPILIN-1 VEGF(XXX)B EXPRESSION BINDING PROTEIN |
| Issue Date | 2013 |
| Publisher | 生物化学与生物物理学研究通讯 |
| Citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2013,441,(1),18-24. |
| Abstract | Vascular endothelial growth factor (VEGF-A) stimulating angiogenesis is required for tumor growth and progression. The conventional VEGF-A isoforms have been considered as pro-angiogenic factors. Another family of VEGF-A isoforms generated by alternative splicing, termed VEGFxxxb isoforms, has anti-angiogenic property, exemplified by VEGF165b. Here, we identify a new number of VEGFxxx family-VEGF111b induced by mitomycin C, although not detected in mitomycin C-unexposed ovarian cancer cells. SKOV3 cells were transfected with pcDNA(3.1) empty vector, pcDNA(3.1)-VEGF111b or pcDNA(3.1)-VEGF165b to collect conditioned mediums respectively. VEGF111b overexpression inhibits proliferation, migration and tube formation of endothelial cell by inhibiting VEGF-R2 phosphorylation and its downstream signaling, similar to VEGF165b but slightly lower than VEGF165b. The anti-angiogenic property depends on the six amino acids of exon 8b of the VEGFxxxb isoforms. Our results show that VEGF111b is a novel potent anti-angiogenic agent that can target the VEGF-R2 and its signaling pathway to inhibit ovarian tumor growth. (C) 2013 Elsevier Inc. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/220134 |
| ISSN | 0006-291X |
| DOI | 10.1016/j.bbrc.2013.09.144 |
| Indexed | SCI(E) |
| Appears in Collections: | 基础医学院 分子心血管学教育部重点实验室 |
