| Title | Simultaneous determination of biotoxins DSP and AZAs in bivalve molluscs and fish by liquid chromatography/tandem mass spectrometry |
| Authors | Zhuo, Liyang Fu, Wusheng Yang, Yiqiang Qiu, Bin Lin, Zhenyu Shan, Liyang Zheng, Lemin Li, Jianrong Chen, Guonan |
| Affiliation | Fuzhou Univ, Key Lab Anal & Detect Technol Food Safety, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ,Dept Chem, Fuzhou 350002, Peoples R China. Qual Inspect Inst Quanzhou, Quanzhou 362000, Fujian, Peoples R China. Fujian Ctr Dis Control & Prevent, Fuzhou 350001, Fujian, Peoples R China. Peking Univ, Key Lab Mol Cardiovasc Sci, Sch Basic Med Sci, Hlth Sci Ctr,Educ Minist,Inst Cardiovasc Sci, Beijing 100191, Peoples R China. Peking Univ, Key Lab Mol Cardiovasc Sci, Sch Basic Med Sci, Inst Syst Biomed,Hlth Sci Ctr,Educ Minist, Beijing 100191, Peoples R China. Minist Hlth, Key Lab Cardiovasc Mol Biol & Regulatory Peptides, Beijing 100191, Peoples R China. Nanjing Med Univ, Lab Human Funct, Atherosclerosis Res Ctr, Nanjing 210029, Jiangsu, Peoples R China. Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Food Safety Key Lab Zhejiang Prov, Hangzhou 310035, Zhejiang, Peoples R China. |
| Keywords | SHELLFISH TOXINS MUSSELS AZASPIRACIDS MS |
| Issue Date | 2014 |
| Publisher | 质谱学快讯 |
| Citation | RAPID COMMUNICATIONS IN MASS SPECTROMETRY.2014,28,(13),1479-1488. |
| Abstract | RATIONALEA method has been developed for simultaneous determination of the toxins OA, DTX-1, AZA-1, AZA-2 and AZA-3 in various aquatic products as these can cause diarrhoetic shellfish poisoning (DSP) in humans, an intoxication characterized by vomiting and diarrhea. METHODSSeparation of the toxins was achieved on a C18 column (150mmx2.1mm, 3.5 mu m) using an acetonitrile/water gradient with formic acid as an eluent modifier. Electrospray ionisation (ESI) in negative mode was used to generate the molecule related ion [M-H]-, for OA and DTX-1, while ESI in positive mode was used to generate the molecule related ion [M+H]+ for AZAs. Samples were extracted with 80% methanol, followed by partitioning with ethyl acetate, purified on a Poly-Sery MAX cartridge and finally analyzed by LC/ESI-MS/MS in the multiple reaction monitoring (MRM) mode. RESULTSThe limit of detection (LOD) and limit of qualification (LOQ) of the method were in the range of 0.02-0.79 mu g/kg and 0.07-2.64 mu g/kg in Scomberomorus niphonius, blood clam and oyster, respectively, recoveries of the toxins at three fortification levels ranged from 71.3% to 104.8% with relative standard deviation from 1.0% to 12.5%. The calibration curves were well linear between the LC peak area of the selected ion pair and the concentration of the toxins, with the correlation coefficient over 0.99. CONCLUSIONSThe method was sufficiently sensitive to permit the determination of the toxins DSP and AZA in sea food. Copyright (c) 2014 John Wiley & Sons, Ltd. |
| URI | http://hdl.handle.net/20.500.11897/212331 |
| ISSN | 0951-4198 |
| DOI | 10.1002/rcm.6900 |
| Indexed | SCI(E) |
| Appears in Collections: | 分子心血管学教育部重点实验室 |
