| Title | Targeted Imaging and Proteomic Analysis of Tumor-Associated Glycans in Living Animals |
| Authors | Xie, Ran Dong, Lu Huang, Rongbing Hong, Senlian Lei, Ruoxing Chen, Xing |
| Affiliation | Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn Synthet, Beijing Natl Lab Mol Sci,Minist Educ, Beijing 100871, Peoples R China. Peking Univ, Funct Biomol Ctr, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China. |
| Keywords | click chemistry integrin ligands liposomes sialic acids tumor imaging CELL-SURFACE SIALYLATION O-LINKED GLYCOSYLATION IN-VIVO FLUORESCENT PROTEINS CLICK CHEMISTRY SIALIC-ACID CANCER INFLAMMATION METASTASIS EXPRESSION |
| Issue Date | 2014 |
| Publisher | angewandte chemie international edition |
| Citation | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION.2014,53,(51),14082-14086. |
| Abstract | Although it has been well known that dynamic changes in glycosylation are associated with tumor progression, it remains challenging to selectively visualize the cancer glycome in vivo. Herein, a strategy for the targeted imaging of tumor-associated glycans by using ligand-targeted liposomes encapsulating azidosugars is described. The intravenously injected liposomal nanoparticles selectively bound to the cancer-cell-specific receptors and installed azides into the melanoma glycans in a xenograft mouse model in a tissue-specific manner. Subsequently, a copper-free click reaction was performed in vivo to chemoselectively conjugate the azides with a near-infrared fluorescent dye. The glycosylation dynamics during tumor growth were monitored by in vivo fluorescence imaging. Furthermore, the newly synthesized sialylated glycoproteins were enriched during tumor growth and identified by glycoproteomics. Compared with the labeling methods using free azidosugars, this method offers improved labeling efficiency and high specificity and should facilitate the elucidation of the functional role of glycans in cancer biology. |
| URI | http://hdl.handle.net/20.500.11897/206932 |
| ISSN | 1433-7851 |
| DOI | 10.1002/anie.201408442 |
| Indexed | SCI(E) |
| Appears in Collections: | å å¦ä¸ å å å·¥ç¨ å¦é ¢ 生命科学学院 生物有机与分子工程教育部重点实验室 |
