| Title | Adenovirus-mediated PDCD5 gene transfer sensitizes K562 cells to apoptosis induced by idarubicin in vitro and in vivo |
| Authors | Ruan, Guo-Rui Zhao, Hong-Shan Chang, Yan Li, Jin-Lan Qin, Ya-Zhen Liu, Yan-Rong Chen, Shan-Shan Huang, Xiao-Jun |
| Affiliation | Peking Univ, Peoples Hosp, Beijing 100044, Peoples R China. Inst Hematol, Beijing 100044, Peoples R China. Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China. Peking Univ, Peoples Hosp, 11 Xizhimen S St, Beijing 100044, Peoples R China. |
| Keywords | PDCD5 gene therapy adenoviral vector idarubicin leukemia nude mice TOPOISOMERASE-II EXPRESSION PROFILES BREAST-CANCER TUMOR-CELLS THERAPY LEUKEMIA MODEL INHIBITORS GROWTH TFAR19 |
| Issue Date | 2008 |
| Publisher | apoptosis |
| Citation | APOPTOSIS.2008,13,(5),641-648. |
| Abstract | PDCD5 (programmed cell death 5) accelerates apoptosis of certain tumor cells and is expressed at low levels in marrow-nucleated cells of AML and CML patients. In the present study, we evaluated the effects of PDCD5 overexpression on drug sensitivity of leukemia cells. K562 cells were treated with idarubicin (IDR) alone or in combination with adenoviral vectors expressing PDCD5 (Ad-PDCD5). As shown by annexin-V-FITC/PI dual labeling, apoptosis rates were markedly increased after combined treatment with Ad-PDCD5 compared to IDR treatment alone. We observed that PDCD5 overexpression significantly improves the antitumor effects of low dose IDR treatment in vivo. Tumor sizes were significantly decreased in combined Ad-PDCD5 and low dose IDR treatment groups compared with single IDR treatment groups. Similar results were obtained with combined systemic treatment of Ad-PDCD5 and low dose IDR, and combined treatment with Ad-PDCD5 local injection and low dose IDR i.p. injection. These results indicate that Ad-PDCD5 may be a promising agent for enhancing chemosensitivity. |
| URI | http://hdl.handle.net/20.500.11897/199179 |
| ISSN | 1360-8185 |
| DOI | 10.1007/s10495-008-0206-9 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 人民医院 |
