| Title | GM1 up-regulates ubiquilin 1 expression in human neuroblastoma cells and rat cortical neurons |
| Authors | Liu, Zhonghua Ruan, Yanyan Yue, Weihua Zhu, Zhihui Hartmann, Tobias Beyreuther, Konrad Zhang, Dai |
| Affiliation | Peking Univ, Inst Mental Hlth, Dept Biochem, Beijing 100871, Peoples R China. Univ Heidelberg, ZMBH Ctr Mol Biol Heidelberg, D-6900 Heidelberg, Germany. 51 Hua Yuan Bei Rd, Beijing 100083, Peoples R China. |
| Keywords | ubiquilin 1 GM I ganglioside Alzheimer's disease gene expression suppression subtractive hybridization AMYLOID PRECURSOR PROTEIN ALZHEIMERS-DISEASE BETA-PROTEIN GROWTH-FACTOR BRAIN INCREASES ACCUMULATION PROTEOLYSIS PEPTIDE |
| Issue Date | 2006 |
| Publisher | 神经科学快报 |
| Citation | NEUROSCIENCE LETTERS.2006,407,(1),59-63. |
| Abstract | GM1 ganglioside was reported to mediate the amyloid beta-protein (A beta) secretion and accumulation in the pathogenesis of Alzheimer's disease (AD). The objective of this project was to comprehend the underlying molecular changes related to amyloid beta-protein precursor (APP) processing pathway induced by GM1. Using suppression subtractive hybridisation (SSH), we detected one prominent sequence with increased expression in human neuroblastoma cells that stably transfected with human APP695 cDNA treated with GM1. This transcript has high identity to human Ubiquilin 1 gene. Differential expression was initially confirmed by dot blot hybridization. This result was further authenticated with quantitative real-time polymerase chain reaction (RT-PCR) analysis. Furthermore, using Western blots, we discovered that GM1 stimulated the expression of Ubiquilin 1 in human neuroblastoma cells and rat cortical neurons while other gangliosides Asialo-GM1 and GD1b did not. Ubiquilin 1 is one of the candidate genes of AD, which have been shown to modulate the gamma-secretase components in the proteolytic processing of APP, and is therefore a putative candidate for further investigation of GM1 mechanisms in the etiology and pathology of AD. (c) 2006 Elsevier Ireland Ltd. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/198725 |
| ISSN | 0304-3940 |
| DOI | 10.1016/j.neulet.2006.08.005 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 第六医院 |
