| Title | Expression of mouse membrane-associated prostaglandin E-2 synthase-2 (mPGES-2) along the urogenital tract |
| Authors | Yang, Guangrui Chen, Lihong Zhang, Yahua Zhang, Xiaoyan Wu, Jing Li, Shuo Wei, Mingfen Zhang, Zhiwen Breyer, Matthew D. Guan, Youfei |
| Affiliation | Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China. Peking Univ, Ctr Diabet, Beijing 100083, Peoples R China. Vanderbilt Univ, Med Ctr, Div Nephrol, Nashville, TN 37232 USA. Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, 38 Xueyuan Rd,Haidian Dist, Beijing 100083, Peoples R China. |
| Keywords | prostaglandin E-2 membrane-associated PGE(2) synthase-2 PGES gene expression urogenital system RAT PERITONEAL-MACROPHAGES RECEPTOR SUBTYPE EP2 PORCINE LUTEAL CELLS GENE-EXPRESSION MICE LACKING CYCLOOXYGENASE ISOZYMES PERIIMPLANTATION PERIOD REDUCED FERTILITY COLLECTING DUCT PGE SYNTHASE-1 |
| Issue Date | 2006 |
| Publisher | biochimica et biophysica acta molecular and cell biology of lipids |
| Citation | BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS.2006,1761,(12),1459-1468. |
| Abstract | Prostaglandin E-2 (PGE(2)) is the most common prostanoid and has a variety of bioactivities including a crucial role in urogenital function. Multiple enzymes are involved in its biosynthesis. Among 3 PGE(2) terminal synthetic enzymes, membrane-associated PGE(2) synthase-2 (mPGES-2) is the most recently identified, and its role remains uncharacterized. In previous studies, membrane-associated PGE(2) synthase-1 (mPGES-1) and cytosolic PGE(2) synthase (OGES) were reported to be expressed along the urogenital tracts. Here we report the genomic structure and tissue distribution of mPGES-2 in the urogenital system. Analysis of several bioinformatic databases demonstrated that mouse mPGES-2 spans 7 kb and consists of 7 exons. The mPGES-2 promoter contains multiple Sp1 sites and a GC box without a TATA box motif Real-time quantitative PCR revealed that constitutive mPGES-2 mRNA was most abundant in the heart, brain, kidney and small intestine. In the urogenital system, mPGES-2 was highly expressed in the renal cortex, followed by the renal medulla and ovary, with lower levels in the ureter, bladder and uterus. Immunohistochemistry studies indicated that mPGES-2 was ubiquitously expressed along the nephron, with much lower levels in the glomeruli. In the ureter and bladder, mPGES-2 was mainly localized to the urothelium. In the reproductive system, mPGES-2 was restricted to the epithelial cells of the testis, epididymis, vas deferens and seminal vesicle in males, and oocytes, stroma cells and corpus luteum of the ovary and epithelial cells of the oviduct and uterus in females. This expression pattern is consistent with an important role for mPGES-2-mediated PGE(2) in urogenital function. (c) 2006 Elsevier B.V. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/198641 |
| ISSN | 1388-1981 |
| DOI | 10.1016/j.bbalip.2006.06.018 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 医学部待认领 |
