| Title | Effects of glucose, insulin, and supernatant from pancreatic beta-cells on brain-pancreas relative protein in rat hippocampus |
| Authors | Lin, Yan-Hua Westenbroek, Christel Tie, Lu Liu, Ai-Hua Yu, He-Ming Ter Horst, Gert J. Li, Xue-Jun |
| Affiliation | Peking Univ, Hlth Sci Ctr, Dept Pharmacol, Sch Basic Med Sci, Beijing 100083, Peoples R China. Peking Univ, Hlth Sci Ctr, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China. Univ Groningen, Dept Psychiat, Grad Sch Behav & Cognit Neurosci, NL-9700 RB Groningen, Netherlands. Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China. Natl Res Inst Family Planning, Beijing 100081, Peoples R China. Peking Univ, Hlth Sci Ctr, Dept Pharmacol, Sch Basic Med Sci, Xueyuan Rd 38, Beijing 100083, Peoples R China. |
| Keywords | brain-pancreas relative protein pancreatic beta-cells hippocampal neurons insulin glucose diabetes CENTRAL-NERVOUS-SYSTEM DIABETIC CHINESE-HAMSTER RECEPTORS CNS PHOSPHORYLATION LOCALIZATION HYPOGLYCEMIA SURVIVAL MELLITUS LESIONS |
| Issue Date | 2006 |
| Publisher | 神经化学研究 |
| Citation | NEUROCHEMICAL RESEARCH.2006,31,(12),1417-1424. |
| Abstract | Brain-pancreas relative protein (BPRP) is a novel protein that mainly expresses in brain and pancreas. In our previous study, we found that various stressors significantly decreased the expression of BPRP in pancreas in vivo, accompanied by changes in insulin and glucose levels, and that expression of BPRP in pancreas also decreased significantly in diabetic rats induced by Streptozocin (STZ). All these findings suggest that BPRP may be a glucose or insulin-sensitive protein. However, how the changes in insulin or glucose levels influence the expression of BPRP in hippocampus requires further study. Here, we investigated the effects of insulin or glucose on the expression of BPRP in primary cultured hippocampal neurons. We supplied hippocampal neurons with glucose, insulin, or supernatant from pancreatic beta-cells, which secrete insulin into the supernatant. Our data showed that insulin had beneficial effect on the viability while no significant effect on the expression of BPRP in hippocampal neurons. On the contrary, 40 mM glucose or free glucose culture significantly decreased the expression of BPRP, while had no significant effect on the viability and apoptosis of hippocampal neurons. Further study showed that levels of insulin in the supernatant collected from pancreatic beta-cells medium changed over days, and that supernatant increased the viability of hippocampal neurons, while it had no obvious effect on the expression of BPRP in hippocampal neurons. These results suggest that BPRP may be a glucose-sensitive protein. |
| URI | http://hdl.handle.net/20.500.11897/198609 |
| ISSN | 0364-3190 |
| DOI | 10.1007/s11064-006-9193-9 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 天然药物与仿生药物国家重点实验室 药学院 |
