| Title | The transport of alpha(1A)-adrenergic receptor with 33-nm step size in live cells |
| Authors | Liang, Zhang-Yi Xu, Ning Guan, Ying-Hua Xu, Ming He, Qi-Hua Han, Qi-De Zhang, You-Yi Zhao, Xin-Sheng |
| Affiliation | Peking Univ, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100871, Peoples R China. Peking Univ, Dept Biol Chem, Coll Chem & Mol Engn, Beijing 100871, Peoples R China. Peking Univ Third Hosp, Inst Vasc Med, Beijing 100083, Peoples R China. Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China. Peking Univ, Ctr Med Anal, Hlth Sci Ctr, Beijing 100083, Peoples R China. |
| Keywords | alpha(1A)-adrenergic receptor transport living cell actin real time single particle tracking myosin PROTEIN-COUPLED RECEPTORS HAND-OVER-HAND MYOSIN-V ALPHA(1)-ADRENOCEPTOR SUBTYPES MEDIATED INTERNALIZATION ACTIN ALPHA(1B)-ADRENOCEPTOR DESENSITIZATION LOCALIZATION MECHANISM |
| Issue Date | 2007 |
| Publisher | 生物化学与生物物理学研究通讯 |
| Citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2007,353,(2),231-237. |
| Abstract | We used the technique of single particle tracking (SPT) with high tempo-spatial resolution to efficiently explore the route and mechanism for the transport Of alpha(1A)-adrenergic receptor (alpha(1A)-AR) in real time in living cells. We found that the initial transport Of alpha(1A)-AR in cells depended on actin filaments with the velocity of 0.2 mu m/s and exhibited discrete 33-nm steps. It was noted that the step size, the rate constant, and the velocities were in accordance with the character of single myosin in vitro, implying that while transporting each endosome myosins did not work in the "tug-of-war" mode and that they did not adopt the strategy to boost up transporting speed by working coordinately. These results provided insight into the mechanism of GPCR transport ill vivo. (c) 2006 Elsevier Inc. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/198543 |
| ISSN | 0006-291X |
| DOI | 10.1016/j.bbrc.2006.11.116 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | å å¦ä¸ å å å·¥ç¨ å¦é ¢ 第三医院 |
