| Title | Alterations of NR2B and PSD-95 expression after early-life epileptiform discharges in developing neurons |
| Authors | Jiang, Qian Wang, Jingmin Wu, Xiru Jiang, Yuwu |
| Affiliation | Peking Univ, Dept Pediat, Beijing 10034, Peoples R China. |
| Keywords | epileptiform discharges development cortical neurons NMDAR subunits PSD-95 protein expression ASPARTATE RECEPTOR SUBTYPES SUBUNIT-SPECIFIC ANTIBODIES EARLY-ONSET EPILEPSY NMDA-RECEPTOR DEVELOPING BRAIN RAT-BRAIN POSTSYNAPTIC DENSITY-95 ETHANOL SENSITIVITY NEONATAL SEIZURES MESSENGER-RNA |
| Issue Date | 2007 |
| Publisher | 国际发育神经科学 |
| Citation | INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE.2007,25,(3),165-170. |
| Abstract | As an extreme form of abnormally synchronized activity, epilepsy may modify patterns of organization in the nervous system. It is clear that enhanced glutamatergic excitatory synaptic transmission with alterations in the expression of ionotropic glutamate receptors is a mechanism critical for seizure susceptibility and excitotoxicity. However, the exact quomodo and the roles of regulated N-methyl-D-aspartate receptor (NMDAR) composition and expression of a major postsynaptic density (PSD) scaffolding molecule, PSD-95, are as yet unclear. To study protein expression changes after epileptiform discharges in cultured immature rat cortical neurons, we divided cells into three groups which were transiently exposed to regular Neurobasal/B27 (control group), physiological solution (PS group) and magnesium-free physiological solution (MGF group) at cultured day 6. Neurons at three different culture ages (DIV7, DIV12 and DIV17) were collected for immunoblotting analysis. We found a decrease in expression of NR2B NMDAR subunit and PSD-95 (P < 0.05) shortly after insult (within 24 h), which may show that brief magnesium-free media treatment of primary cultured rat cortical neurons, an in vitro model of seizure brain injury, has a major influence on the expression of NR2B subunit and PSD-95. (C) 2007 ISDN. Published by Elsevier Ltd. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/198433 |
| ISSN | 0736-5748 |
| DOI | 10.1016/j.ijdevneu.2007.02.001 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 待认领 |
