| Title | Analysis of meiotic segregation patterns and interchromosomal effects in sperm from six males with Robertsonian translocations |
| Authors | Chen, Yongjian Huang, Jin Liu, Ping Qiao, Jie |
| Affiliation | Peking Univ, Ctr Reprod Med, Hosp 3, Beijing 100083, Peoples R China. |
| Keywords | fluorescence in-situ hybridization interchromosomal effects meiotic segregation Robertsonian translocation sperm INFERTILE MEN FISH ANALYSIS CARRIER SPERMATOZOA CHROMOSOMES NUCLEI ABNORMALITIES ASSOCIATION TRIVALENT PROBES |
| Issue Date | 2007 |
| Publisher | journal of assisted reproduction and genetics |
| Citation | JOURNAL OF ASSISTED REPRODUCTION AND GENETICS.2007,24,(9),406-411. |
| Abstract | Purpose To provide more genetic information about meiotic segregation behavior and the possibility of interchromosomal effects (ICE) in spermatozoa from carriers of Robertsonian (Rob) translocations. Materials and methods Meiotic segregation behavior in spermatozoa from six carriers of Rob translocations, four t(13;14), one t(14;22) and one t(13;21), was investigated by dual fluorescence in-situ hybridization (FISH). Aneuploidy for chromosomes 18, X and Y was studied by triple FISH. Results The rate of normal/balanced spermatozoa resulting from alternate segregation ranged from 78.14 to 86.88%. The frequency of unbalanced spermatozoa resulting from adjacent segregation varied between 11.70 and 19.53%. The higher frequencies of aneuploidy for sex chromosome were observed in three Rob translocation carriers. In addition, the increased rates of diploid were found in two t(13;14) carriers. Conclusions Alternate segregation is dominant in the different types of Rob translocations. Some carriers may be at an increased risk for ICE. |
| URI | http://hdl.handle.net/20.500.11897/198004 |
| ISSN | 1058-0468 |
| DOI | 10.1007/s10815-007-9137-6 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 第三医院 |
