| Title | Applications of novel monoclonal antibodies specific for synuclein-gamma in evaluating its levels in sera and cancer tissues from colorectal cancer patients |
| Authors | Liu, Caiyun Guo, Jianping Qu, Like Bing, Dong Meng, Lin Wu, Jian Shou, Chengchao |
| Affiliation | Peking Univ, Key Lab Minist Educat Canc Res & Applicat, Dept Biochem & Mol Biol, Sch Oncol,Beijing Canc Hosp & Inst, Beijing 100036, Peoples R China. Peking Univ, Dept Pathol, Sch Oncol, Beijing Canc Hosp & Inst, Beijing 100036, Peoples R China. Peking Univ, Key Lab Minist Educat Canc Res & Applicat, Dept Biochem & Mol Biol, Sch Oncol,Beijing Canc Hosp & Inst, 52 Fucheng Rd, Beijing 100036, Peoples R China. |
| Keywords | synuclein-gamma monoclonal antibody colorectal cancer immunoassay GENE CPG ISLAND BREAST-CANCER ALPHA-SYNUCLEIN PARKINSONS-DISEASE HUMAN PLASMA EXPRESSION CELLS LOCALIZATION PROMOTES IDENTIFICATION |
| Issue Date | 2008 |
| Publisher | cancer letters |
| Citation | CANCER LETTERS.2008,269,(1),148-158. |
| Abstract | Overexpressions of synuclein-gamma (SNCG) in different cancers display stage-specific patterns. At present, appropriate anti-SNCG monoclonal antibodies (mAbs) with high specificity and affinity are unavailable for different immunoassays in clinical applications. In this study, we generated 10 mAbs against endogenous SNCG and evaluated SNCG levels in several colorectal cancer cell lines, serum samples and tumor tissues from colorectal cancer (CRC) patients. Elevated SNCG levels in cancer cell lines evaluated by a novel sandwich ELISA were consistent with data obtained from Western blot. Secreted SNCG protein levels in sera from CRC patients could be detected by the sandwich ELISA and were further confirmed by Western blot analysis following SNCG enrichment. Immunohistochemical results showed that SNCG was highly expressed in tumor cells of CRC patients, but was undetectable in the adjacent normal epithelium. Taken together, these novel anti-SNCG mAbs specifically recognized endogenous SNCG and were suitable for measuring SNCG levels in cell lysates, human serum samples, and tumor tissues. Elevated serum SNCG and overexpressed SNCG in tumor tissue from CRC patients suggest SNCG is a potential biomarker for CRC. (C) 2008 Elsevier Ireland Ltd. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/197911 |
| ISSN | 0304-3835 |
| DOI | 10.1016/j.canlet.2008.04.037 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 北京肿瘤医院 |
