| Title | Ectopic expression of angiopoietin-1 promotes neuronal differentiation in neural progenitor cells through the Akt pathway |
| Authors | Bai, Yun Cui, Ming Meng, Zhijun Shen, Li He, Qihua Zhang, Xiaoyan Chen, Fengrong Xiao, Junjun |
| Affiliation | Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Cell Biol, Beijing 100191, Peoples R China. Peking Univ, Hosp 3, Dept Cardiol, Beijing 100191, Peoples R China. Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Cell Biol, 38 Xueyuan Rd, Beijing 100191, Peoples R China. |
| Keywords | Angiopoetin-1 Neural progenitor cells Neuronal differentiation Neurogenesis Tie2 Akt ENDOTHELIAL GROWTH-FACTOR CENTRAL-NERVOUS-SYSTEM NEUROGENESIS SURVIVAL ISCHEMIA PROLIFERATION APOPTOSIS OUTGROWTH PROTECTS RECEPTOR |
| Issue Date | 2009 |
| Publisher | 生物化学与生物物理学研究通讯 |
| Citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2009,378,(2),296-301. |
| Abstract | In regions of adult neurogenesis, neural progenitor cells (NPCs) are found in close proximity to blood vessels within a so-called 'vascular niche'. Neurogenesis is linked to angiogenesis via certain growth factors. We propose that angiopoietin-1 (Ang1), which is similar to VEGF, has a unique role in neurogenesis independent of its role in angiogenesis. In this study, primary cultures of NPCs were transduced with recombinant: adenoviruses expressing Ang1 and induced to differentiate With dibutyryl cyclic AMP (dbcAMP). Neuronal differentiation was evaluated by quantitative PCR, immunofluorescence microscopy and Western blot analysis. The results show that ectopic expression of Ang1 promotes neuronal differentiation and neurite outgrowth in NPCs, while this effect was blocked by the presence of anti-Tie2 receptor antibody or the P13-K inhibitor, LY294002. Our results Suggest that Ang1, identified originally as in angiogenic factor, can also stimulate in vitro neurogenesis in NPCs through the Akt pathway. (c) 2008 Elsevier Inc. All fights reserved. |
| URI | http://hdl.handle.net/20.500.11897/197379 |
| ISSN | 0006-291X |
| DOI | 10.1016/j.bbrc.2008.11.052 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 基础医学院 第三医院 |
