| Title | Effects of Lipid Composition and Phase on the Membrane Interaction of the Prion Peptide 106-126 Amide |
| Authors | Zhong, Jian Yang, Chunhui Zheng, Wenfu Huang, Lixin Hong, Yuankai Wang, Lijun Sha, Yinlin |
| Affiliation | Peking Univ, Biomed X Ctr, Sch Basic Med Sci, Dept Biophys,Single Mol & Nanobiol Lab, Beijing 100083, Peoples R China. Peking Univ, Ctr Prot Sci, Beijing 100083, Peoples R China. Univ Coll Dublin, Dublin 2, Ireland. |
| Keywords | ATOMIC-FORCE MICROSCOPY MODEL MEMBRANES TRITON X-100 ANTIMICROBIAL PEPTIDES ALZHEIMERS-DISEASE SUPPORTED BILAYERS PROTEIN-FRAGMENT AMYLOID PEPTIDE CELL-MEMBRANES RAFT FORMATION |
| Issue Date | 2009 |
| Publisher | biophysical journal |
| Citation | BIOPHYSICAL JOURNAL.2009,96,(11),4610-4621. |
| Abstract | Lipid rafts are specialized liquid-ordered (L-o) phases of the cell membrane that are enriched in sphingolipids and cholesterol (Chl), and surrounded by a liquid-disordered (L-d) phase enriched in glycerophospholipids. Lipid rafts are involved in the generation of pathological forms of proteins that are associated with neurodegenerative diseases. To investigate the effects of lipid composition and phase on the generation of pathological forms of proteins, we constructed an L-d-gel phase-separated 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)/sphingomyelin (from bovine brain (BSM))-supported lipid bilayer (SLB) and an L-d-L-o phase-separated POPC/BSM/Chl SLB. We used in situ time-lapse atomic force microscopy to study the interactions between these SLBs and the prion peptide K(106)TNMKHMAGAAAAGAVVGGLG(126) (PrP106-126) amide, numbered according to the human prion-peptide sequence. Our results show that: 1), with the presence of BSM in the L-d phase, the PrP106-126 amide induces fully penetrated porations in the L-d phase of POPC/BSM SLB and POPC/BSM/Chl SLB; 2), with the presence of both BSM and Chl in the L-d phase, the PrP106-126 amide induces the disintegration of the L-d phase of POPC/BSM/Chl SLB; and 3), with the presence of both BSM and Chl in the L-o phase, PrP106-126 amide induces membrane thinning in the L-o phase of POPC/BSM/Chl SLB. These results provide comprehensive insight into the process by which the PrP106-126 amide interacts with lipid membranes. |
| URI | http://hdl.handle.net/20.500.11897/197230 |
| ISSN | 0006-3495 |
| DOI | 10.1016/j.bpj.2009.01.036 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 基础医学院 |
