| Title | The role of calcium ions in the interactions of PrP106-126 amide with model membranes |
| Authors | Zhong, Jian Yang, Chunhui Zheng, Wenfu Huang, Lixin Hong, Yuankai Wang, Lijun Sha, Yinlin |
| Affiliation | Peking Univ, Dept Biophys, Singlemol & Nanobiol Lab, Sch Basic Med Sci, Beijing 100191, Peoples R China. Peking Univ, Biomed X Ctr, Beijing 100191, Peoples R China. Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China. |
| Keywords | Ca2+ PrP106-126 amide Poration Membrane disintegration Atomic force microscopy ATOMIC-FORCE MICROSCOPY SUPPORTED LIPID-BILAYERS PRION PROTEIN-FRAGMENT SURFACE-PLASMON RESONANCE ANTIMICROBIAL PEPTIDES ELECTROLYTE-SOLUTIONS ALKALINE-PHOSPHATASE MOLECULAR-MECHANISM HYDRATION FORCES CELLS |
| Issue Date | 2010 |
| Publisher | colloids and surfaces b biointerfaces |
| Citation | COLLOIDS AND SURFACES B-BIOINTERFACES.2010,77,(1),40-46. |
| Abstract | In this work, we investigated the interactions of PrP106-126 amide with 1-palmitoyl-2-oleoyl-3-phosphocholine (POPC) and POPC/bovine brain sphingomyelin (BSM) membranes in the presence of calcium ions by in situ time-lapse atomic force microscopy (AFM) and circular dichroism (CD). The CD results show that Ca2+ has no obvious effects on the random coil conformation of PrP106-126 amide. The tapping mode AFM results demonstrate that electrostatic interaction decreases the measured heights of supported lipid bilayers (SLBs) in HBS-Ca2+ solution. Electrostatic interaction analysis also can be used to determine the applied force in liquid tapping mode AFM. The interactions of PrP106-126 amide with membranes by AFM demonstrate the following: (i)Ca2+ inhibits the interaction of PrP106-126 amide with POPC lipid and (ii) the co-interaction between Ca2+ and BSM increases the poration ability of PrP106-126 amide. These results imply that the main role of Ca2+ in the interactions of PrP106-126 amide with membranes is changing the surface properties of the membranes. (C) 2010 Elsevier B.V. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/196929 |
| ISSN | 0927-7765 |
| DOI | 10.1016/j.colsurfb.2010.01.001 |
| Indexed | SCI(E) EI PubMed |
| Appears in Collections: | 基础医学院 |
