| Title | Identification of differentially expressed transcripts and translatants targeted by knock-down of endogenous PCBP1 |
| Authors | Huo, Li-Rong Ju, Weina Yan, Ming Zou, Jun-Hua Yan, Wu He, Bing Zhao, Xin-Liang Jenkins, Edmund C. Brown, W. Ted Zhong, Nanbert |
| Affiliation | New York State Inst Basic Res Dev Disabil, Dept Human Genet, Staten Isl, NY 10314 USA. Peking Univ, Ctr Med Genet, Beijing 100083, Peoples R China. New York State Inst Basic Res Dev Disabil, Dept Human Genet, 1050 Forest Hill Rd, Staten Isl, NY 10314 USA. |
| Keywords | PCBP1 RNA interference Transcriptional profile Translational profile Histone proteins POLY-C BINDING-PROTEIN-1 MESSENGER-RNA 3'-UNTRANSLATED REGION SIGNAL-TRANSDUCTION MOLECULAR-BASIS PROTEINS BINDING SUPPRESSION CANCER CELLS |
| Issue Date | 2010 |
| Publisher | biochimica et biophysica acta proteins and proteomics |
| Citation | BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS.2010,1804,(10),1954-1964. |
| Abstract | PCBP1 is a member of the hnRNP family and participates in the regulation of transcription and translation. Previously, we identified transcripts targeted by overexpression of exogenous PCBP1. To further determine if these altered transcripts may also be targeted by a lack of PCBP1, we depleted endogenous PCBP1 in human SH-SY5Y cells. We identified 941 transcripts with the Affymetrix and 1362 with the Agilent expression platforms. There were 375 transcripts identified by both platforms, including 328 down-regulated and 47 up-regulated. The identified transcripts could be grouped into neuronal, cell signaling, metabolic, developmental, and differentiation categories, with pathway involvement in Wnt signaling, TGF beta signaling, translation factors and nuclear receptors. A proteomic profiling study with a two-dimensional chromatographic platform showed global translational changes over a range of isoelectric points (pI) = 4.84-8.42. This study identifies the transcripts affected by knock-down of endogenous PCBP1 and compares them to the transcripts affected by overexpression of PCBP1. (C) 2010 Elsevier B.V. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/195769 |
| ISSN | 1570-9639 |
| DOI | 10.1016/j.bbapap.2010.07.002 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 医学部待认领 |
