| Title | Role of vesicle pools in action potential pattern-dependent dopamine overflow in rat striatum in vivo |
| Authors | Wang, Shi-Rong Yao, Wei Huang, Hong-Ping Zhang, Bo Zuo, Pan-Li Sun, Liang Dou, Hai-Qiang Li, Qing Kang, Xin-Jiang Xu, Hua-Dong Hu, Mei-Qin Jin, Mu Zhang, Lei Mu, Yu Peng, Ji-Yun Zhang, Claire Xi Ding, Jiu-Ping Li, Bao-Ming Zhou, Zhuan |
| Affiliation | Peking Univ, Inst Mol Med, State Key Lab Membrane Bioengn, Beijing 100871, Peoples R China. Huazhong Univ Sci & Technol, Sch Life Sci, Inst Biochem & Biophys, Wuhan 430074, Peoples R China. Fudan Univ, Inst Neurobiol, Inst Brain Sci, Shanghai 200433, Peoples R China. Fudan Univ, State Key Lab Med Neurobiol, Inst Brain Sci, Shanghai 200433, Peoples R China. Peking Univ, Inst Mol Med, State Key Lab Membrane Bioengn, 5 Yiheyuan Rd, Beijing 100871, Peoples R China. |
| Keywords | action potential pattern amperometry dopamine in vivo striatum vesicle release ADRENAL CHROMAFFIN CELLS TRANSMITTER RELEASE IMPULSE FLOW AMPEROMETRIC DETECTION NONLINEAR RELATIONSHIP NEUROENDOCRINE CELLS QUANTAL SECRETION FOREBRAIN-BUNDLE NERVE-TERMINALS CENTRAL SYNAPSE |
| Issue Date | 2011 |
| Publisher | 神经化学杂志 |
| Citation | JOURNAL OF NEUROCHEMISTRY.2011,119,(2),342-353. |
| Abstract | Action potential (AP) patterns and dopamine (DA) release are known to correlate with rewarding behaviors, but how codes of AP bursts translate into DA release in vivo remains elusive. Here, a given AP pattern was defined by four codes, termed total AP number, frequency, number of AP bursts, and inter-burst time [N, f, b, i].. The 'burst effect' was calculated by the ratio (gamma) of DA overflow by multiple bursts to that of a single burst when total AP number was fixed. By stimulating the medial forebrain bundle using AP codes at either physiological (20 Hz) or supraphysiological (80 Hz) frequencies, we found that DA was released from two kinetically distinct vesicle pools, the fast-releasable pool (FRP) and prolonged-releasable pool (PRP), in striatal dopaminergic terminals in vivo. We examined the effects of vesicle pools on AP-pattern dependent DA overflow and found, with given 'burst codes' [b = 8, i = 0.5 s], a large total AP number [N = 768, f = 80 Hz] produced a facilitating burst-effect (gamma[b8/b1] = 126 +/- 3%), while a small total AP number [N = 96, 80 Hz] triggered a depressing-burst-effect (gamma[b8/b1] = 29 +/- 4%). Furthermore, we found that the PRP (but not the FRP) predominantly contributed to the facilitating-burst-effect and the FRP played an important role in the depressing-burst effect. Thus, our results suggest that striatal DA release captures pre-synaptic AP pattern information through different releasable pools. |
| URI | http://hdl.handle.net/20.500.11897/192914 |
| ISSN | 0022-3042 |
| DOI | 10.1111/j.1471-4159.2011.07440.x |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 分子医学研究所 |
