| Title | Expression of immunoglobulin G in esophageal squamous cell carcinomas and its association with tumor grade and Ki67 |
| Authors | Zhang, Liying Hu, Shengping Korteweg, Christine Chen, Zhengshan Qiu, Yamei Su, Min Gu, Jiang |
| Affiliation | Shantou Univ, Coll Med, Dept Pathol, Shantou 515031, Peoples R China. Peking Beijing Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100191, Peoples R China. Shantou Univ, Coll Med, Ctr Mol Biol, Shantou 515031, Peoples R China. Shantou Univ, Coll Med, Dept Pathol, 22 Xinling Rd, Shantou 515031, Peoples R China. |
| Keywords | Immunoglobulin G Esophageal cancer Recombination-activating genes 1 and 2 Ki67 Tumor grade MULTIPLE MANAGEMENT MODALITIES EPITHELIAL CANCER-CELLS CHAIN CONSTANT-REGION NEONATAL FC-RECEPTOR V(D)J RECOMBINATION GENE TRANSCRIPTS GAMMA-GLOBULIN MAMMARY-GLAND LINES SPECIFICITY |
| Issue Date | 2012 |
| Publisher | human pathology |
| Citation | HUMAN PATHOLOGY.2012,43,(3),423-434. |
| Abstract | We and other research groups have previously shown that various cancer types can express immunoglobulin G, but investigation on of immunoglobulin G expression in esophageal cancer, a highly malignant tumor, and its biological significance has been lacking. In this study, we examined immunoglobulin G protein and its messenger RNA, as well as the expressions of recombination-activating gene 1, recombination-activating gene 2, and activation-induced cytidine deaminase in 142 cases of esophageal cancer tissues, and 2 esophageal cancer cell lines (Eca109,SHEEC). We also compared their expressions with tumor grade and a proliferation marker, Ki67. We used immunohistochemistry, immunofluorescence, in situ hybridization, laser microdissection coupled with reverse transcriptase polymerase chain reaction, and Western blot analysis. We detected transcripts of immunoglobulin G 1 heavy-chain constant region, immunoglobulin-kappa and lambda-light chains, immunoglobulin G variable region, and recombination-activating genes 1 and 2 in both esophageal cancer tissues and cell lines, whereas activation-induced cytidine deaminase was not detected. No immunoglobulin G receptor subtypes were detected. Statistic analysis revealed that immunoglobulin G expression con-elated well with tumor grades (P < .001) mid with the proliferation marker Ki67 (P < .001). Our results indicate that human esophageal cancer cells are capable of synthesizing immunoglobulin G, which is likely involved in the growth and proliferation of this highly malignant cancer and might also be used as a prognostic indicator in esophageal squamous cell carcinomas. (C) 2012 Elsevier Inc. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/192910 |
| ISSN | 0046-8177 |
| DOI | 10.1016/j.humpath.2011.05.020 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 医学部待认领 |
