| Title | Comparative evaluation of the teratogenicity of genistein and genistin using rat whole embryo culture and limbud micromass culture methods |
| Authors | Zou, Peng Xing, Lina Tang, Qiuqiong Liu, Ran Hao, Weidong |
| Affiliation | Peking Univ, Dept Toxicol, Sch Publ Hlth, Beijing Key Lab Toxicol Res & Risk Assessment Foo, Beijing 100191, Peoples R China. Peking Univ, Dept Toxicol, Sch Publ Hlth, Beijing Key Lab Toxicol Res & Risk Assessment Foo, 38 Xueyuan Rd, Beijing 100191, Peoples R China. |
| Keywords | Genistein Genistin Micromass culture Teratogenicity Whole embryo culture SPRAGUE-DAWLEY RATS ISOFLAVONE GENISTEIN DIETARY CANCER BIOAVAILABILITY PHYTOESTROGENS METABOLITES TOXICITY WOMEN |
| Issue Date | 2012 |
| Publisher | 食品与化学品毒理学 |
| Citation | FOOD AND CHEMICAL TOXICOLOGY.2012,50,(8),2831-2836. |
| Abstract | Genistein (GEN) is one kind of phytoestrogen. Several studies have demonstrated the teratogenic potential of GEN in vitro by postimplantation rat whole embryo culture (WEC) assay, but GEN showed no teratogenic effects in vivo even at a dose up to 1000 mg/kg bw/day. The mechanism of such discrepancy is still unclear. Because more than 80% of total genistein (free plus glycoside form) in circulation is its glycoside metabolite, genistin (GIN), we thus hypothesize that genistin is non-teratogenic. To prove this hypothesis, rat whole embryo culture (WEC) and limbud micromass culture methods were applied to compare the teratogenic effects of GEN and GIN on developing embryos in vitro. In WEC assay, we found that the development of embryos was affected by GEN treatment dose-dependently, while GIN-treated embryos displayed slight developmental defects only at the highest dose (222 mu M). In micromass culture assay, the IC50 of cell proliferation and differentiation for GEN were 15.6 and 37.2 mu M, respectively, while neither was influenced by GIN treatment up to 111 mu M. Collectively, our study indicated that GEN showed no teratogenic effects in vivo probably due to its transformation to the non-teratogenic metabolite, GIN. (C) 2012 Elsevier Ltd. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/192241 |
| ISSN | 0278-6915 |
| DOI | 10.1016/j.fct.2012.05.009 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 公共卫生学院 |
