Title | Nicotine dynamically modulates dopamine clearance in rat striatum in vivo |
Authors | Guo, Ning Yao, Wei Wang, Shi-Rong Zhu, Jun Huang, Dong Zuo, Pan-Li Kang, Xin-Jiang Fu, Chong-Luo Zhou, Zhuan Zhang, Bo |
Affiliation | Peking Univ, Inst Mol Med, State Key Lab Membrane Bioengn, Beijing 100871, Peoples R China. Peking Univ, Inst Mol Med, Ctr Life Sci, Beijing 100871, Peoples R China. Liaocheng Univ, Coll Life Sci, Liaocheng 252059, Shandong, Peoples R China. Peking Univ, Inst Mol Med, State Key Lab Membrane Bioengn, 5 Yiheyuan Rd, Beijing 100871, Peoples R China. |
Keywords | Dopamine Nicotine Striatum Dopamine transporter Uptake MEDIAL PREFRONTAL CORTEX NUCLEUS-ACCUMBENS TRANSPORTER FUNCTION RELEASE TRAFFICKING RECEPTOR COCAINE OVERFLOW CELLS |
Issue Date | 2012 |
Publisher | 国际神经化学 |
Citation | NEUROCHEMISTRY INTERNATIONAL.2012,60,(4),355-359. |
Abstract | Nicotine binds to nicotinic acetylcholine receptors on dopaminergic terminals to evoke dopamine (DA) release. The clearance of released DA occurs rapidly through reuptake into nerve terminals through the DA transporter (DAT). However, whether nicotine modulates DAT function in vivo is still not well understood. In the present study, we determined the effect of nicotine on DA clearance using in vivo amperometric recording in the striatum of urethane-anesthetized rats. Stable DA release was evoked by electrical stimulation of the medial forebrain bundle (MFB). Subsequently, nicotine or saline was administered with MFB stimulation at 10-min intervals for 60 min. Kinetic analysis revealed that nicotine decreased the amplitude of DA overflow and the maximal DA clearance rate (V-max) in response to stimulation of 96 pulses at 80 Hz. Surprisingly, nicotine enhanced the maximal DA clearance rate (V-max) by stimulation of 768 pulses at 80 Hz. Furthermore, we found that this paradoxical effect of nicotine on V-max depended on the stimulation pattern. These results suggest that nicotine may exert its addictive role by dynamically modulating DAT function in vivo. Published by Elsevier Ltd. |
URI | http://hdl.handle.net/20.500.11897/191652 |
ISSN | 0197-0186 |
DOI | 10.1016/j.neuint.2012.01.022 |
Indexed | SCI(E) PubMed |
Appears in Collections: | 分子医学研究所 |