| Title | Impulse-dependent extracellular resting dopamine concentration in rat striatum in vivo |
| Authors | Zuo, Pan-Li Yao, Wei Sun, Liang Kuo, Shu-Ting Li, Qing Wang, Shi-Rong Dou, Hai-Qiang Xu, Hua-Dong Zhang, Claire Xi Kang, Xin-Jiang Zhou, Zhuan Zhang, Bo |
| Affiliation | Peking Univ, Inst Mol Med, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100871, Peoples R China. Peking Univ, Inst Mol Med, Ctr Life Sci, Beijing 100871, Peoples R China. Shandong Univ, Sch Med, Inst Physiol, Jinan 250012, Shandong, Peoples R China. Peking Univ, Inst Mol Med, 5 Yiheyuan Rd, Beijing 100871, Peoples R China. |
| Keywords | Resting dopamine Undershoot In vivo Amperometry Striatum D2 receptors CARBON-FIBER MICROELECTRODES NUCLEUS-ACCUMBENS QUANTITATIVE MICRODIALYSIS INVIVO VOLTAMMETRY CAUDATE-PUTAMEN RELEASE MODULATION NEURONS TRANSPORTER GLUTAMATE |
| Issue Date | 2013 |
| Publisher | 国际神经化学 |
| Citation | NEUROCHEMISTRY INTERNATIONAL.2013,62,(1),50-57. |
| Abstract | The ambient resting dopamine (DA) concentration in brain regulates cognition and motivation. Despite its importance, resting DA level in vivo remains elusive. Here, by high-frequency stimulation of the medial forebrain bundle and immediately following the stimulus-induced DA overflow, we recorded a DA "undershoot" which is a temporal reduction of DA concentration to a level below the baseline. Based on the DA undershoot, we predicted a resting DA concentration of similar to 73 nM in rat striatum in vivo. Simulation studies suggested that removing basal DA by DAT during the post-stimulation inhibition of tonic DA release caused the DA undershoot, and the resting concentration of DA modulated the kinetics of the evoked DA transient. The DA undershoot was eliminated by either blocking D2 receptors with haloperidol or blocking the DA transporter (DAT) with cocaine. Therefore, the impulse-dependent resting DA concentration is in the tens of nanomolar range and is modulated by the presynaptic D2 receptors and the DAT in vivo. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/190781 |
| ISSN | 0197-0186 |
| DOI | 10.1016/j.neuint.2012.11.006 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 分子医学研究所 |
