| Title | Eif3ba regulates cranial neural crest development by modulating p53 in zebrafish |
| Authors | Xia, Zhidan Tong, Xiangjun Liang, Fang Zhang, Yihan Kuok, Chikin Zhang, Yingla Liu, Xinxing Zhu, Zuoyan Lin, Shuo Zhang, Bo |
| Affiliation | Peking Univ, Key Lab Cell Proliferat & Differentiat, Minist Educ, Coll Life Sci, Beijing 100871, Peoples R China. Peking Univ, Sch Chem Biol & Biotechnol, Lab Chem Genom, Shenzhen Grad Sch, Shenzhen 518055, Peoples R China. Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA USA. |
| Keywords | Cranial neural crest Cardiac neural crest eif3ba nrp2b p53 Zebrafish CARDIAC OUTFLOW TRACT CRANIOFACIAL DEVELOPMENT HEART DEVELOPMENT LEOPARD-SYNDROME CELL-MIGRATION GENE-FUNCTION SURVIVAL LINEAGE EMBRYOS DIFFERENTIATION |
| Issue Date | 2013 |
| Publisher | developmental biology |
| Citation | DEVELOPMENTAL BIOLOGY.2013,381,(1),83-96. |
| Abstract | Congenital diseases caused by abnormal development of the cranial neural crest usually present craniofacial malformations and heart defects while the precise mechanism is not fully understood. Here, we show that the zebrafish eif3ba mutant caused by pseudo-typed retrovirus insertion exhibited a similar phenotype due to the hypogenesis of cranial neural crest cells (NCCs). The derivatives of cranial NCCs, including the NCC-derived cell population of pharyngeal arches, craniofacial cartilage, pigment cells and the myocardium derived from cardiac NCCs, were affected in this mutant. The expression of several neural crest marker genes, including crestin, dlx2a and nrp2b, was specifically reduced in the cranial regions of the eif3ba mutant. Through fluorescence-tracing of the cranial NCC migration marker nrp2b, we observed reduced intensity of NCC-derived cells in the heart. In addition, p53 was markedly up-regulated in the eif3ba mutant embryos, which correlated with pronounced apoptosis in the cranial area as shown by TUNEL staining. These findings suggest a novel function of eif3ba during embryonic development and a novel level of regulation in the process of cranial NCC development, in addition to providing a potential animal model to mimic congenital diseases due to cranial NCC defects. Furthermore, we report the identification of a novel transgenic fish line Et(gata2a:EGFP)(pku418) to trace the migration of cranial NCCs (including cardiac NCCs); this may serve as an invaluable tool for investigating the development and dynamics of cranial NCCs during zebrafish embryogenesis. (C) 2013 Elsevier Inc. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/190502 |
| ISSN | 0012-1606 |
| DOI | 10.1016/j.ydbio.2013.06.009 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 生命科学学院 化学生物学与生物技术学院 细胞增殖分化调控机理研究教育部重点实验室 |
