| Title | Improved detection of human papillomavirus harbored in healthy skin with FAP6085/64 primers |
| Authors | Li, Jingjing Pan, YaQi Xu, Zhongyao Wang, Qiyan Hang, Dong Shen, Na Liu, Mengfei Zhang, Chanyuan Abliz, Amir Deng, Qiuju Cai, Hong Ke, Yang |
| Affiliation | Peking Univ, Canc Hosp & Inst, Minist Educ, Key Lab Carcinogenesis & Translat Res, Beijing, Peoples R China. |
| Keywords | Cutaneous HPV Healthy skin FAP cross primers Large samples CUTANEOUS HUMAN PAPILLOMAVIRUSES PCR DNA DIVERSITY LESIONS SPECTRUM WARTS ASSAY BETA |
| Issue Date | 2013 |
| Publisher | 病毒学方法杂志 |
| Citation | JOURNAL OF VIROLOGICAL METHODS.2013,193,(2),633-638. |
| Abstract | FAP59/64, FAP6085/6319, and CUT primer sets were designed for detecting cutaneous HPV and have been used in many clinical and epidemiology studies. The FAP6085/64 primer set was first evaluated in this study and the FAP6085/64 combination was found to be much more sensitive than all three original primer sets by using HPV plasmids as a template. To confirm further the effectiveness of the FAP6085/64 primer set in human DNA templates, 90 palmar exfoliated cell DNA samples were used to detect the cutaneous HPV by both the FAP59/64 and FAP6085/64 primer sets. The overall proportion of HPV detection in those skin samples was 77.8% (70/90) using FAP6085/64, as compared to 55.6% (50/90) using FAP59/64. The FAP6085/64 primer set was also applied in a population based study. The proportion of HPV detection was 73.96% (2076/2807) in skin samples collected from healthy individuals, and a total of 336 different PV types were found. Sixty (17.9%) of them were fully characterized HPV types, 127 (37.8%) were putative HPV types which had been described previously, 149 (44.3%) were novel putative HPV types, and two animal PVs were also detected. These results suggest that the FAP6085/64 primer set was sensitive and effective for detection of cutaneous HPV in healthy skin samples. (C) 2013 Published by Elsevier B.V. |
| URI | http://hdl.handle.net/20.500.11897/190443 |
| ISSN | 0166-0934 |
| DOI | 10.1016/j.jviromet.2013.06.026 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 北京肿瘤医院 |
