| Title | Expression, regulation and roles of miR-26a and MEG3 in tongue squamous cell carcinoma |
| Authors | Jia, Ling-Fei Wei, Su-Bi Gan, Ye-Hua Guo, Yong Gong, Kai Mitchelson, Keith Cheng, Jing Yu, Guang-Yan |
| Affiliation | Peking Univ, Dept Oral & Maxillofacial Surg, Sch & Hosp Stomatol, Beijing 100081, Peoples R China. Tsinghua Univ, Med Syst Biol Res Ctr, Beijing 100084, Peoples R China. Peking Univ, Mol Biol Lab, Sch & Hosp Stomatol, Beijing 100081, Peoples R China. Tsinghua Univ, Sch Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China. Peking Univ, Dept Oral & Maxillofacial Surg, Sch & Hosp Stomatol, 22 Zhongguancun Ave South, Beijing 100081, Peoples R China. |
| Keywords | miR-26a lncRNA MEG3 DNMT3B squamous cell carcinoma LONG NONCODING RNA HEPATOCELLULAR-CARCINOMA MICRORNA EXPRESSION BREAST-CANCER LIVER-CANCER EZH2 APOPTOSIS PATHWAY GROWTH GENE |
| Issue Date | 2014 |
| Publisher | international journal of cancer |
| Citation | INTERNATIONAL JOURNAL OF CANCER.2014,135,(10),2282-2293. |
| Abstract | MicroRNA miR-26a and long noncoding RNA (lncRNA) MEG3 gene have been independently reported to be tumor suppressor genes in various cancers, but neither has been previously associated with tongue squamous cell carcinoma (TSCC). We report here that miR-26a and lncRNA MEG3 gene expression were both strongly reduced in TSCC compared with levels in matched nonmalignant tissues, and combined low expression levels of both miR-26a and MEG3 emerged as an independent prognostic factor for poor clinical outcome in TSCC patients. Assays in the human TSCC cell lines SCC-15 and CAL27 showed that miR-26a targets the DNA methyltransferase 3B transcript and that its inhibition may result in the upregulation of MEG3, providing a plausible link between the observed reduction of miR-26a and MEG3 in TSCC tissue. Furthermore, the overexpression of miR-26a or MEG3 in SCC-15 and CAL27 cells inhibited cell proliferation and cell cycle progression, and promoted cell apoptosis. Considering the poor prognostic outcomes associated with reduced miR-26a and MEG3, our findings imply that these factors likely play important antitumor effects in TSCC pathogenesis. Furthermore, they represent potential prognostic biomarkers for stratification of TSCC patients. |
| URI | http://hdl.handle.net/20.500.11897/190094 |
| ISSN | 0020-7136 |
| DOI | 10.1002/ijc.28667 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 口腔医院 |
