| Title | Excessive Intrauterine Fluid Cause Aberrant Implantation and Pregnancy Outcome in Mice |
| Authors | Lu, Shan Peng, Hongying Zhang, He Zhang, Li Cao, Qichen Li, Rong Zhang, Ying Yan, Liying Duan, Enkui Qiao, Jie |
| Affiliation | Peking Univ, Dept Obstet & Gynecol, Hosp 3, Beijing 100871, Peoples R China. Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China. Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing, Peoples R China. Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China. |
| Keywords | IN-VITRO FERTILIZATION UTERINE LUMINAL FLUID EMBRYO IMPLANTATION ENDOMETRIAL RECEPTIVITY ASSISTED REPRODUCTION HYDROSALPINX IVF SALPINGECTOMY EXPRESSION WOMEN |
| Issue Date | 2013 |
| Publisher | plos one |
| Citation | PLOS ONE.2013,8,(10). |
| Abstract | The normal intrauterine fluid environment is essential for embryo implantation. In hydrosalpinx patients, the implantation and pregnancy rates are markedly decreased after IVF-embryo transfer, while salpingectomy could significantly improve the pregnancy rates. The leakage of hydrosalpinx fluid into the endometrial cavity was supposed to be the major cause for impaired fertility. However, the underlying mechanisms of hydrosalpinx fluids on implantation and ongoing pregnancy were not fully understood and remain controversial regarding its toxicity. In present study, by infusing different volume of non-toxic fluid (0.9% saline) into uterine lumen before embryo implantation in mice (Day4 08: 30), we found that while the embryos were not "flushed out" from the uteri, the timing of implantation was deferred and normal intrauterine distribution (embryo spacing) was disrupted. The abnormal implantation at early pregnancy further lead to embryo growth retardation, miscarriage and increased pregnancy loss, which is similar to the adverse effects observed in hydrosalpinx patients undergoing IVF-ET. We further examined uterine receptivity related gene expression reported to be involved in human hydrosalpinx (Lif, Hoxa10, Integrin a(v) and beta(3)). The results showed that expression of integrin alpha(v) and beta(3) were increased in the fluid infused mouse uteri, implicating a compensatory effect to cope with the excessive fluid environment. Our data suggested that the adverse effects of excessive non-toxic luminal fluid on pregnancy are primarily due to the mechanical interference for normal timing and location of embryo apposition, which might be the major cause of decreased implantation rate in IVF-ET patients with hydrosalpinx. |
| URI | http://hdl.handle.net/20.500.11897/189852 |
| ISSN | 1932-6203 |
| DOI | 10.1371/journal.pone.0078446 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 第三医院 |
