| Title | Microchip-based human serum atherogenic lipoprotein profile analysis |
| Authors | Wang, Hua Zhang, Wei Wan, Jun Liu, Weiwei Yu, Bo Jin, Qinghui Guan, Ming |
| Affiliation | Fudan Univ, Shanghai Med Coll, Huashan Hosp, Dept Lab Med & Cent Lab, Shanghai 200040, Peoples R China. Fudan Univ, Shanghai Med Coll, Huashan Hosp, Cent Lab, Shanghai 200040, Peoples R China. Shenzhen PKU HKUST Med Ctr, Biomed Res Inst, Shenzhen 518036, Guangdong, Peoples R China. Tongji Univ, Shanghai Peoples Hosp 10, Dept Lab Med, Shanghai 200072, Peoples R China. Shanghai Skin Dis Hosp, Dept Lab Med, Shanghai 200071, Peoples R China. Peking Univ, Shenzhen Hosp, Dept Dermatol, Shenzhen 518036, Guangdong, Peoples R China. Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, Shanghai 200050, Peoples R China. |
| Keywords | Microchip Gold nanoparticles NBD C-6-ceramide Atherogenic lipoprotein profile LOW-DENSITY-LIPOPROTEIN CAPILLARY-ZONE-ELECTROPHORESIS HEART-DISEASE RISK SEPARATION LDL ATHEROSCLEROSIS PHENOTYPE MARKER ASSAY VLDL |
| Issue Date | 2014 |
| Publisher | 分析生物化学 |
| Citation | ANALYTICAL BIOCHEMISTRY.2014,467,75-83. |
| Abstract | Owing to the mounting evidence of serum lipid changes in atherosclerosis, there has been increasing interest in developing new methods for analyzing atherogenic lipoprotein profiles. The separation of lipoprotein and lipoprotein subclasses has been demonstrated using a microchip capillary electrophoresis (CE) system [Chromatographia 74 (2011) 799-805]. In contrast to this previous study, the current report demonstrates that sdLDL peak efficiencies can be improved dramatically by adding gold nanoparticles (AuNPs) to the sample. Moreover, NBD C-6-ceramide was identified as a satisfactory dye for specific labeling and quantitation of individual serum lipoproteins. The accuracy of the method was evaluated by comparison with ultracentrifuge separated small, dense, low-density lipoprotein (sdLDL). A high correlation was observed between these two methods for sdLDL cholesterol. Lipid levels were investigated between atherosclerotic patients and healthy controls. The variation of serum atherogenic lipoprotein profiles for atherosclerotic patients pre- and post-treatment was assessed by microchip CE. This method has potential for the rapid and sensitive detection of different lipoprotein classes as well as their subclasses and, therefore, is suitable for routine clinical applications. Microchip-based atherogenic lipoprotein profile assays will greatly improve the analysis of risk factors in atherosclerosis and will provide useful information for monitoring the effect of therapies on atherosclerotic disease. (C) 2014 Elsevier Inc. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/189142 |
| ISSN | 0003-2697 |
| DOI | 10.1016/j.ab.2014.08.031 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 深圳医院 |
