| Title | Live-cell and super-resolution imaging reveal that the distribution of wall-associated protein A is correlated with the cell chain integrity of Streptococcus mutans |
| Authors | Li Yongliang Liu Zhen Zhang Yifei Su Qian P Xue Boxin Shao Shipeng Zhu Yun Xu Xiao Wei Shicheng Sun Yujie |
| Affiliation | Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, School and Hospital of Stomatology, Peking University, Beijing, 100081, China. |
| Keywords | Streptococcus mutans,Biofilm,cell wall anchored proteins,dynamic distribution,live cell imaging,super-resolution imaging,wall associated protein A (WapA) |
| Issue Date | 2015 |
| Publisher | molecular oral microbiology |
| Citation | Molecular oral microbiology.2015. |
| Abstract | Streptococcus mutans (S. mutans) is a primary pathogen responsible for dental caries. S. mutans has an outstanding ability to form biofilm, which is vital for virulence. Previous studies have shown that knockout of Wall-associated protein A (WapA) affects cell chain and biofilm formation of S. mutans. However, as a surface protein, the distribution of WapA remains unknown, but it is important to understand the mechanism underlying the function of WapA. This study applied the fluorescence protein mCherry as a reporter gene to characterise the dynamic distribution of WapA in S. mutans via time lapse and super-resolution fluorescence imaging. The results revealed interesting subcellular distribution patterns of WapA in single, dividing, and long chains of S. mutans cells. It appears at the middle of the cell and moves to the poles as the cell grows and divides. In a cell chain, after each round of cell division, such dynamic relocation results in WapA distribution at the previous cell division sites, resulting in a pattern where WapA is located at the boundary of two adjacent cell pairs. This WapA distribution pattern corresponds to the breaking segmentation of wapA deletion cell chains. The dynamic relocation of WapA through the cell cycle increases our understanding of the mechanism of wapA in maintaining cell chain integrity and biofilm formation. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/188051 |
| ISSN | 2041-1014 |
| DOI | 10.1111/omi.12100 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 口腔医院 生命科学学院 |
