| Title | The Arabidopsis APC4 subunit of the anaphase-promoting complex/cyclosome (APC/C) is critical for both female gametogenesis and embryogenesis |
| Authors | Wang, Yanbing Hou, Yingnan Gu, Hongya Kang, Dingming Chen, Zhangliang Liu, Jingjing Qu, Li-Jia |
| Affiliation | Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China. China Agr Univ, Coll Agron & Biotechnol, Beijing 100094, Peoples R China. Natl Plant Gene Res Ctr Beijing, Beijing 100101, Peoples R China. |
| Keywords | anaphase promoting complex subunit 4 anaphase-promoting complex cyclosome female gametogenesis embryogenesis connector auxin DEPENDENT AUXIN GRADIENTS CELL-CYCLE GAMETOPHYTE DEVELOPMENT PLANT EMBRYOGENESIS FISSION YEAST HOBBIT GENE COMPLEX EMBRYO MITOSIS DEGRADATION |
| Issue Date | 2012 |
| Publisher | plant journal |
| Citation | PLANT JOURNAL.2012,69,(2),227-240. |
| Abstract | The anaphase-promoting complex/cyclosome (APC/C) is an E3 ubiquitin ligase that is involved in regulating cell-cycle progression. It has been widely studied in yeast and animal cells, but the function and regulation of the APC/C in plant cells are largely unknown. The Arabidopsis APC/C comprises at least 11 subunits, only a few of which have been studied in detail. APC4 is proposed to be a connector in the APC/C in yeast and animals. Here, we report the functional characterization of the Arabidopsis APC4 protein. We examined three heterozygous plant lines carrying apc4 alleles. These plants showed pleiotropic developmental defects in reproductive processes, including abnormal nuclear behavior in the developing embryo sac and aberrant cell division in embryos; these phenotypes differ from those reported for mutants of other subunits. Some ovules and embryos of apc4/+ plants also accumulated cyclin B protein, a known substrate of APC/C, suggesting a compromised function of APC/C. Arabidopsis APC4 was expressed in meristematic cells of seedlings, ovules in pistils and embryos in siliques, and was mainly localized in the nucleus. Additionally, the distribution of auxin was distorted in some embryos of apc4/+ plants. Our results indicate that Arabidopsis APC4 plays critical roles in female gametogenesis and embryogenesis, possibly as a connector in APC/C, and that regulation of auxin distribution may be involved in these processes. |
| URI | http://hdl.handle.net/20.500.11897/162681 |
| ISSN | 0960-7412 |
| DOI | 10.1111/j.1365-313X.2011.04785.x |
| Indexed | SCI(E) EI |
| Appears in Collections: | 生命科学学院 |
