| Title | Anti-C1q autoantibodies from active lupus nephritis patients could inhibit the clearance of apoptotic cells and complement classical pathway activation mediated by C1q in vitro |
| Authors | Pang, Yun Yang, Xiao-Wei Song, Yan Yu, Feng Zhao, Ming-Hui |
| Affiliation | Peking Univ, Div Renal, Dept Med,Minist Educ China,Inst Nephrol, Hosp 1,Key Lab Renal Dis,Minist Hlth China,Key La, Beijing 100034, Peoples R China. Chinese Peoples Liberat Army Gen Hosp, Dept Nephrol, Affiliated Hosp 1, Beijing 100048, Peoples R China. Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China. |
| Keywords | Anti-C1q autoantibodies Apoptosis Complements Lupus nephritis Systemic lupus erythematosus RENAL-DISEASE ACTIVITY COLLAGEN-LIKE REGION IMMUNE-RESPONSES CHINESE PATIENTS SERUM-LEVELS ANTIBODIES ERYTHEMATOSUS IGG MACROPHAGES DEPOSITION |
| Issue Date | 2014 |
| Publisher | immunobiology |
| Citation | IMMUNOBIOLOGY.2014,219,(12),980-989. |
| Abstract | Anti-C1q antibodies are prevalent in patients with active lupus nephritis and were found to be closely associated with renal involvement and predictive for a flare of nephritis. However, the pathogenesis of anti-C1q antibodies involved in human lupus nephritis remains unclear. C1q, which plays a key role in apoptotic cell and immune complex removal, is a very important functional molecule in the pathogenesis of SLE. The aim of this study was to investigate the influence of anti-C1q autoantibodies from active lupus nephritis patients on the bio-functions of C1q in vitro. We purified IgG autoantibodies against C1q from lupus nephritis patients, and found that they could recognize C1q bound on early apoptotic cells at 30 mu g/ml, and could significantly decrease the phagocytosis by macrophages of early apoptotic cells opsonized by 50 mu g/ml C1q in comparison with normal IgG. Levels of circulating immune complexes of the ten patients were measured by a circulating immune complexes (CIC)-C1q Enzyme Immunoassay Kit. Anti-C1q autoantibodies affinity purified by microtiter plates could significantly inhibit the deposition of C3c on CIC-C1q in a dose dependent manner in comparison with IgG from 10 healthy blood donors. The binding of opsonized immune complexes to RBCs was significantly inhibited by anti-C1q autoantibodies purified by microtiter plates in a dose dependent manner. Our observations suggest that serum anti-C1q autoantibodies from active lupus nephritis patients could interfere with some biological function of C1q in vitro. (C) 2014 Elsevier GmbH. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/159870 |
| ISSN | 0171-2985 |
| DOI | 10.1016/j.imbio.2014.07.004 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 第一医院 |
