| Title | Human Gut Microbiota Changes Reveal the Progression of Glucose Intolerance |
| Authors | Zhang, Xiuying Shen, Dongqian Fang, Zhiwei Jie, Zhuye Qiu, Xinmin Zhang, Chunfang Chen, Yingli Ji, Linong |
| Affiliation | Peking Univ, Dept Endocrinol & Metab, Peoples Hosp, Ctr Diabet, Beijing 100871, Peoples R China. BGI Shenzhen, Shenzhen, Peoples R China. Peking Univ, Dept Clin Epidemiol, Peoples Hosp, Beijing 100871, Peoples R China. |
| Keywords | TYPE-2 DIABETES-MELLITUS DIET-INDUCED OBESITY AKKERMANSIA-MUCINIPHILA INSULIN-RESISTANCE METABOLIC SYNDROME RISK-FACTORS BODY-WEIGHT LIFE-STYLE SP NOV. MICE |
| Issue Date | 2013 |
| Publisher | plos one |
| Citation | PLOS ONE.2013,8,(8). |
| Abstract | To explore the relationship of gut microbiota with the development of type 2 diabetes (T2DM), we analyzed 121 subjects who were divided into 3 groups based on their glucose intolerance status: normal glucose tolerance (NGT; n = 44), prediabetes (Pre-DM; n = 64), or newly diagnosed T2DM (n = 13). Gut microbiota characterizations were determined with 16S rDNA-based high-throughput sequencing. T2DM-related dysbiosis was observed, including the separation of microbial communities and a change of alpha diversity between the different glucose intolerance statuses. To assess the correlation between metabolic parameters and microbiota diversity, clinical characteristics were also measured and a significant association between metabolic parameters (FPG, CRP) and gut microbiota was found. In addition, a total of 28 operational taxonomic units (OTUs) were found to be related to T2DM status by the Kruskal-Wallis H test, most of which were enriched in the T2DM group. Butyrate-producing bacteria (e. g. Akkermansia muciniphila ATCCBAA-835, and Faecalibacterium prausnitzii L2-6) had a higher abundance in the NGT group than in the pre-DM group. At genus level, the abundance of Bacteroides in the T2DM group was only half that of the NGT and Pre-DM groups. Previously reported T2DM-related markers were also compared with the data in this study, and some inconsistencies were noted. We found that Verrucomicrobiae may be a potential marker of T2DM as it had a significantly lower abundance in both the pre-DM and T2DM groups. In conclusion, this research provides further evidence of the structural modulation of gut microbiota in the pathogenesis of diabetes. |
| URI | http://hdl.handle.net/20.500.11897/159238 |
| ISSN | 1932-6203 |
| DOI | 10.1371/journal.pone.0071108 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 人民医院 |
