| Title | Absolute quantification of serum microRNA-122 and its correlation with liver inflammation grade and serum alanine aminotransferase in chronic hepatitis C patients |
| Authors | Wang, Jiang-hua Jiang, Dong Rao, Hui-yng Zhao, Jing-min Wang, Yu Wei, Lai |
| Affiliation | Peking Univ, Peoples Hosp, Inst Hepatol, Beijing Key Lab Hepatitis & Immunotherapy Liver D, Beijing 100044, Peoples R China. Chinese Ctr Dis Control & Prevent, Beijing 102206, Peoples R China. 302 Mil Hosp China, Beijing, Peoples R China. Peking Univ, Peoples Hosp, Inst Hepatol, Beijing Key Lab Hepatitis & Immunotherapy Liver D, 11 Xizhimen South St, Beijing 100044, Peoples R China. |
| Keywords | MicroRNA-122 Chronic hepatitis C ALT Liver inflammation CIRCULATING MICRORNAS VIRUS-INFECTION NATURAL-HISTORY INTERFERON THERAPY INJURY MIR-122 BIOMARKER REPLICATION FIBROSIS |
| Issue Date | 2015 |
| Publisher | international journal of infectious diseases |
| Citation | INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES.2015,30,52-56. |
| Abstract | Objectives: MicroRNA-122 has been shown to be crucial for efficient HCV RNA replication in vitro. Pretreatment intrahepatic microRNA-122 levels in chronic hepatitis C (CHC) patients have been associated with the outcomes of interferon therapy. Here, we determined microRNA-122 serum levels in CHC patients and healthy donors using an absolute quantification approach and evaluated the correlation with liver inflammation grades and serum alanine aminotransferase (ALT) levels. Methods: Serum samples were collected from 105 treatment-naive CHC patients, 11 acute hepatitis patients, and 33 healthy donors. Serum microRNA-122 was measured using the TaqMan RT-qPCR. The cycle threshold values were converted to copy numbers by drawing a standard curve using a chemical synthetic standard. For accurate quantification, copy numbers were further normalized according to the recovery ratios of spiked-in cel-miR-39. Results: Serum levels of microRNA-122 were significantly higher in acute hepatitis and CHC patients than in healthy donors (p < 0.001). However, there was no significant association between microRNA-122 and ALT serum levels or liver inflammation grades. Conclusions: The present study showed that serum microRNA-122 was elevated in acute and chronic hepatitis patients. However, this biomarker for acute liver injury did not reflect the liver inflammation activity in CHC patients. (C) 2014 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. |
| URI | http://hdl.handle.net/20.500.11897/159127 |
| ISSN | 1201-9712 |
| DOI | 10.1016/j.ijid.2014.09.020 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 人民医院 |
