| Title | Polyaspartoyl.L-arginine enhances prostacyclin synthesis in rat aortic endothelial cells |
| Authors | Tang, Zhiyu Yang, Jian Liu, Xiaoyan Wang, Yinye Peng, Shiqi |
| Affiliation | Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Beijing 100191, Peoples R China. Capital Univ Med Sci, Beijing Key Labs Hydrone & Peptides, Beijing 100069, Peoples R China. |
| Keywords | Polyaspartoyl.L-arginine Nitric oxide Prostacyclin Rat aortic endothelial cells NITRIC-OXIDE SYNTHASE PLATELET-AGGREGATION RELEASE CYCLOOXYGENASE INHIBITION ACTIVATION MECHANISM CULTURE ASSAY |
| Issue Date | 2008 |
| Publisher | european journal of pharmacology |
| Citation | EUROPEAN JOURNAL OF PHARMACOLOGY.2008,601,(1-3),124-128. |
| Abstract | Nitricoxide (NO) and prostacyclin(PGl(2)) are two of the most important vasodilators produced by endothelial cells, the regulation of NO on PGl(2) production has not been fully clear yet Polyaspartoyl.L-arginine (PDR) is an L-arginine residue-rich compound with inhibitory effects of platelet aggregation and thrombosis. This study investigated its effects on NO production in rat aortic endothelial cells (RAECs) and observed the influence of NO on PGI(2) level in RAECs. NO concentration in the medium of RAECs was assessed with fluorometric method; 6-keto-PGF(1 alpha), the stable metabolite of PGl(2), in the medium of RAECs was measured with radioimmunoassay kits; Protein level of PGl(2) synthase in RAECs was determined by Western blot analysis. PDR (17.0 similar to 170 mu g/ml, equal to 0.5 mu M-5 PM) enhanced NO level in culture medium of RAEC with concentration-dependent manner (P<0.01); L-arginine (170 mu g/ml, equal to 1000 mu M) and 1.70 mu g/ml (0.05 mu M) of PDR slightly increased NO level (P>0.05). Interestingly PDR (1.70-500 mu g/ml), L-arginine (17.0-170 mu g/ml) significantly elevated PGl(2) levels in medium of RAECs (P<0.01), NO synthase inhibitor, N(G)-nitro L-arginine methyl ester (L-NAME), markedly inhibited the elevated PGl(2) levels by PDR and L-arginine. NO donor. sodium nitroprusside(SNP)(1-500 mu M), showed the most powerful effects of increasing PGl(2) level in RAECs, which was not influenced by L-NAME. Cyclooxigenase(COX) inhibitor, indomethacin, significantly reduced elevated PGl(2) level by both PDR and SNP in RAEC medium. PDR (170 mu g/ml) increased the expression of PGl(2) synthase, L-NAME partly inhibited this effect. In conclusion, PDR enhances PGl(2) synthesis in RAEC, which is attributed to its effect of NO production; the stimulating effect of PDR on PGl(2) synthesis may be mediated via COX and PGl(2) synthase. (C) 2008 Published by Elsevier B.V. |
| URI | http://hdl.handle.net/20.500.11897/156839 |
| ISSN | 0014-2999 |
| DOI | 10.1016/j.ejphar.2008.11.003 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 药学院 |
