| Title | Point-of-Care Multiplexed Assays of Nucleic Acids Using Microcapillary-based Loop-Mediated Isothermal Amplification |
| Authors | Zhang, Yi Zhang, Lu Sun, Jiashu Liu, Yulei Ma, Xingjie Cui, Shangjin Ma, Liying Xi, Jianzhong Jeff Jiang, Xingyu |
| Affiliation | Beijing Engn Res Ctr BioNanotechnol, Beijing 100190, Peoples R China. Natl Ctr Nanosci & Technol, Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China. Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China. Chinese Ctr Dis Control & Prevent China CDC, State Key Lab Infect Dis Prevent & Control, Natl Ctr AIDS STD Control & Prevent NCAIDS, Collaborat Innovat Ctr Diag & Treatment Infect Di, Beijing 102206, Peoples R China. CAAS, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Peoples R China. |
| Keywords | POLYMERASE-CHAIN-REACTION CAPILLARY SENSOR ARRAY REVERSE-TRANSCRIPTION MICROFLUIDIC SYSTEMS RAPID DETECTION DNA EXTRACTION DIGITAL PCR LAMP DEVICE ELECTROPHORESIS |
| Issue Date | 2014 |
| Publisher | analytical chemistry |
| Citation | ANALYTICAL CHEMISTRY.2014,86,(14),7057-7062. |
| Abstract | This report demonstrates a straightforward, robust, multiplexed and point-of-care microcapillary-based loop-mediated isothermal amplification (cLAMP) for assaying nucleic acids. This assay integrates capillaries (glass or plastic) to introduce and house sample/reagents, segments of water droplets to prevent contamination, pocket warmers to provide heat, and a hand-held flashlight for a visual readout of the fluorescent signal. The cLAMP system allows the simultaneous detection of two RNA targets of human immunodeficiency virus (HIV) from multiple plasma samples, and achieves a high sensitivity of two copies of standard plasmid. As few nucleic acid detection methods can be wholly independent of external power supply and equipment, our cLAMP holds great promise for point-of-care applications in resource-poor settings. |
| URI | http://hdl.handle.net/20.500.11897/154258 |
| ISSN | 0003-2700 |
| DOI | 10.1021/ac5014332 |
| Indexed | SCI(E) EI |
| Appears in Collections: | 工学院 |
